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https: file. Scan Now! – Upload Your Scan Now! Filehost Download links.. using such a measurement technique to characterize, on a single cell basis, the relative abundance of two populations.
If the copy number of all the important target sequences (for example, the genetic sequences comprising the 16S rDNA) could be controlled, it would be very convenient to be able to determine the percent abundance of one population by measuring the copy number of the other.
In principle, one could vary the copy number of one target molecule and measure the change in the amount of a second target molecule to determine the percent of one population in a sample mixture. However, there are two major shortcomings of this technique. First, the absolute copy number of each target molecule can only be measured if an absolute measurement of a reference molecule is made. Most molecular techniques have not been optimized for absolute measurement, so that such measurements cannot be made. Second, even if the absolute copy number of a target molecule could be determined, the absolute copy number of the reference molecule would then have to be known. This second factor makes a quantitative determination of the relative abundance of a given sample mixture more difficult.
In addition, many molecular techniques such as PCR and hybridization, cannot distinguish between a single molecule and multiple molecules of a given sequence. Consequently, the methods described above cannot be used to determine the relative abundance of one population (nucleic acid) based on the measurement of a change in the level of one type of sequence (DNA or RNA) in a mixture of cells.
Similarly, attempts to estimate the relative abundance of one population in a mixture of cells by determining the copy number of a target sequence are problematic. First, to obtain even a rough estimate of relative abundance, large amounts of starting material (1, 2 or 10,000 cells) would be required to estimate the total cell number by extrapolation. However, it is well known that cells vary in size and therefore present a problem when measuring the amount of starting DNA present in a mixture of cells. Second, while molecular techniques can be optimized for target molecule number measurements, they do not lend themselves to absolute measurement. One consequence of this is that the ratio of a target sequence to a reference sequence can only be measured in a relative sense (percent of target molecules relative to a single reference molecule). Therefore, a convenient method of determining the percent abundance of one population (nucleic acid) in a mixture of cells on a cell by cell basis has http://www.graham-lawler.com/?p=3870

 

NOTES IN BUSINESS LAW

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